Background: India is the highest TB burden country in the world and accounts for nearly one fi fth (20%) of global burden of tuberculosis. Every year, approximately 1.8 million persons develop tuberculosis, of which about 8,00,000 are infectious and until recently, 3,70,000 cases died of it annually. In India, today, two deaths occur every three minutes from tuberculosis, but these deaths can be prevented with proper care and treatment. Materials and Methods: An interventional study was conducted from January to March 2009 by interviewing 86 fi nal year medical students studying at Rural Medical College, Loni, India. A pre-designed and pre-tested questionnaire was used to collect the pre-workshop and post-workshop data. Questions were based on the knowledge regarding tuberculosis. Results were analyzed statistically using percentage, proportions, and Chi-square test. Results: In this study, about 55 (63.9%) participants correctly specifi ed RNTCP objectives in pre-test, while in post-test, 80 (93.1%) participants could state correct response (P<0.0001). In pre-test, 57 (66.3%) participants thought that family member can be a DOTS provider, while during post-test, 80 (93.1%) participants realized that DOTS should not be given by family member (P<0.0001). Similarly, highly signifi cant difference in pre- and post-test was observed for DOTS components. Conclusion: Sensitization workshops increase the knowledge of tuberculosis amongst the medical students. RNTCP training should be a part of regular activity of all medical colleges